Anti-Phospho-Progesterone Receptor (Ser190) antibody (bs-3356R)

Anti-Phospho-Progesterone Receptor (Ser190) antibody

DATASHEET

Host:Rabbit

Target Protein:Phospho-Progesterone Receptor (Ser190)

IR:Immunogen Range:GL(p-S)P

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:25154

Swiss Prot:Q63449

Source:KLH conjugated Synthesised phosphopeptide derived from rat Progesterone Receptor around the phosphorylation site of Ser190:GL(p-S)P 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboJNC terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER alpha and ER beta. ER alpha and ER beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-Aand hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1ug/Test)

Cross Reactive Species:Human
Mouse
Rat
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Uterus (Mouse) Lysate at 40 ug
Lane 2: Testis (Mouse) Lysate at 40 ug
Lane 3: Testis (Rat) Lysate at 40 ug
Lane 4: MCF-7 (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Lane 6: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-progesterone receptor (bs-3356R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118/82-88 kD
Observed band size: 120 kD

Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
Lane 2: Uterus (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-Progesterone Receptor (Ser190) (bs-3356R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110 kD
Observed band size: 110 kD