Anti-Phospho-SHP2 (Tyr580) antibody
| 英文名称 | Phospho-SHP2 (Tyr580) |
| 中文名称 | 磷酸化蛋白酪氨酸磷酸酶2抗体 |
| 别 名 | SHP2 (phospho Y580); p-SHP2 (phospho Y580); PTPN11(Phospho-Tyr580); PTPN11; PTN11_HUMAN; BPTP 3; BPTP3; CFC; MGC14433; SHP 2; Noonan syndrome 1; Noonan syndrome 1 protein tyrosine phosphatase 2C; NS 1; NS1; Protein tyrosine phosphatase 2C; Protein Tyrosine Phosphatase Non receptor Type 11; PTP 1D; PTP 2C; PTP1D; PTP2C; PTPN 11; SAP2; SH PTP2; SH PTP3; SH2 domain containing protein tyrosine phosphatase 2; SHIP2; SHP 2; SHP-2; SHPTP 2; SHPTP2; SHPTP3; SIT protein precursor; Syp; Tyrosine protein phosphatase non receptor type 11; Src homology 2 (SH2) domain containing phosphotyrosinephosphatase 2. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-SHP2 (Tyr580)
IR:Immunogen Range:RV(p-Y)E
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5781
Swiss Prot:Q06124
Source:KLH conjugated Synthesised phosphopeptide derived from human PTPN11 around the phosphorylation site of Tyr584:RV(p-Y)E
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane1: Brain(Mouse) lysate at 30ug;
Lane2: Muscle(Mouse) lysate at 30ug;
Primary: Anti-Phospho-SHP2 (bs-3405R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1:5000 dilution;
Predicted band size: 66 kD
Observed band size: 66 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-SHP2 (Tyr580)) Polyclonal Antibody, Unconjugated (bs-3405R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-SHP2 (Tyr580)) Polyclonal Antibody, Unconjugated (bs-3405R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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