Anti-CYP11A1 antibody
| 英文名称 | CYP11A1 |
| 中文名称 | 细胞色素P450 11A1抗体 |
| 别 名 | Cholesterol 20 22 desmolase； Cholesterol desmolase； Cholesterol monooJNCgenase (side chain cleaving)； Cholesterol side chain cleavage enzyme； Cholesterol side chain cleavage enzyme mitochondrial； Cholesterol side-chain cleavage enzyme； CP11A_HUMAN； CYP11A； CYP11A1； CYPXIA1； Cytochrome P450 11A1； Cytochrome P450 11A1 mitochondrial； Cytochrome P450 family 11 subfamily A polypeptide 1； Cytochrome P450 subfamily XIA； Cytochrome P450(scc)； Cytochrome P450C11A1； mitochondrial； P450SCC； Steroid 20 22 lyase. |
DATASHEET
Host:Rabbit
Target Protein:CYP11A1
IR:Immunogen Range:321-420/521
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1583
Swiss Prot:P05108
Source:KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC:321-420/521
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooJNCgenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Placenta (Mouse) Lysate at 30 ug
Primary: Anti- CYP11A1 (Ser576) (bs-3608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 53/57 kD
Observed band size: 57 kD
Sample:
Lane1: Brain(Rat) Lysate at 30 ug
Lane2: Kidney(Rat)Lysate at 30 ug
Primary: Anti-CYP11A1/P450SCC (bs-3608R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 53kD
Observed band size : 53kD
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (bs-3608R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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