Anti-Phospho-Vimentin (Ser56) antibody (bs-3471R)

Anti-Phospho-Vimentin (Ser56) antibody

DATASHEET

Host:Rabbit

Target Protein:Phospho-Vimentin (Ser56)

IR:Immunogen Range:S(p-S)PG

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7431

Swiss Prot:P08670

Source:KLH conjugated Synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser56:S(p-S)PG 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(0.2μg /Test)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Sheep
Guinea Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Kidney (Mouse) Lysate at 40 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 5: Spleen (Mouse) Lysate at 40 ug
Lane 6: Lymph node (Mouse) Lysate at 40 ug
Lane 7: Testis (Mouse) Lysate at 40 ug
Lane 8: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 9: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-Vimentin (Ser56) (bs-3471R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

Sample:
Cerebrum(Mouse) Lysate at 40 ug
Kidney(Mouse) Lysate at 40 ug
Primary: Anti-Phospho-Vimentin (Ser56) (bs-3471R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 53 kD