Anti-Phospho-ZAP70 (Tyr315 + Tyr319) antibody
| 英文名称 | Phospho-ZAP70 (Tyr315 + Tyr319) |
| 中文名称 | 磷酸化zeta相关蛋白70抗体 |
| 别 名 | Zap-70(Phospho-Tyr315/Tyr319); Zap-70(Phospho-Y315/Y319); p-ZAP70 (Tyr315/Tyr319); p-ZAP-70 (Tyr315/Tyr319); p-ZAP70 (Y315/Y319); ZAP-70; ZAP 70; ZAP70; zeta-associated protein 70; ZAP-70=protein tyrosine kinase Syk homolog {SH2-like and C-terminal kinase domains}; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-associated protein; Syk-related tyrosine kinase. SRK; STD; TZK; ZAP70_HUMAN; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-ZAP70 (Tyr315 + Tyr319)
IR:Immunogen Range:SV(p-Y)ESP(p-Y)SD
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:7535
Swiss Prot:P43403
Source:KLH conjugated Synthesised phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyr315/Tyr319:SV(p-Y)ESP(p-Y)SD
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-F(1:100-500)
Flow-Cyt(1ug/test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Chicken
Dog
Pig
Cow
Horse
Rabbit
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-ZAP70 (Tyr315 + Tyr319) (bs-3478R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZAP-70 Antibody(bs-3478R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-Phospho-ZAP70 (Tyr315 + Tyr319) antibody (bs-3478R)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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