Anti-Nox1 antibody (bs-3682R)

Anti-Nox1 antibody 

DATASHEET

Host:Rabbit

Target Protein:Nox1

IR:Immunogen Range:425-515/515

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:27035

Swiss Prot:Q9Y5S8

Source:KLH conjugated synthetic peptide derived from human Nox1:425-515/515 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oJNCgen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-Nox1 (bs-3682R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD

Sample:
Testis (Mouse) Lysate at 40 ug
Uterus (Mouse) Lysate at 40 ug
DU145(Human) Cell Lysate at 30 ug
Primary: Anti- Nox1 (bs-3682R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 60 kD

Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti- Nox1 (bs-3682R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 62 kD
Observed band size: 57 kD