Anti-GDF15 antibody
| 英文名称 | GDF15 |
| 中文名称 | 生长分化因子15/巨嗜细胞抑制因子1抗体 |
| 别 名 | GDF 15; GDF-15; GDF15_HUMAN; Growth differentiation factor 15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC 1; Mic-1; MIC1; NAG 1; NAG-1; NAG1; NRG 1; NRG-1; NRG1; NSAID (nonsteroidal anti inflammatory drug) activated protein 1; NSAID; NSAID regulated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PDF; PLAB; Placental bone morphogenetic protein; Placental bone morphogenic protein; Placental TGF beta; Placental TGF-beta; Prostate differentiation factor; PTGF beta; PTGFB. |
DATASHEET
Host:Rabbit
Target Protein:GDF15
IR:Immunogen Range:221-308/308
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:9518
Swiss Prot:Q99988
Source:KLH conjugated synthetic peptide derived from human GDF-15:221-308/308
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Growth differentiation factor 15 (GDF15) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. It has been implicated in a variety of functions directly related to tumorigenicity including antiproliferative and pro-apoptotic effects. BMP proteins are secreted growth factors that are characterized by seven conserved cysteine residues. In general, they are regulators of cell growth and differentiation in both embryonic and adult tissues. GDF15 is an important downstream mediator of DNA damage signaling and a transcriptional target of p53.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Urinary bladder (Mouse) Lysate at 40 ug
Lane 2: Large intestine (Mouse) Lysate at 40 ug
Lane 3: Urinary bladder (Rat) Lysate at 40 ug
Lane 4: Large intestine (Rat) Lysate at 40 ug
Lane 5: HT1080 (Human) cell Lysate at 40 ug
Lane 6: HepG2 (Human) cell Lysate at 40 ug
Lane 7: K562 (Human) cell Lysate at 40 ug
Lane 8: A549 (Human) cell Lysate at 40 ug
Primary: Anti-GDF15 (bs-3818R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
Sample:
K562 Cell Lysate at 30 ug
A431 Cell Lysate at 30 ug
Primary: Anti-GDF15 (bs-3818R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:34 kD
Observed band size:30 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (GDF15) Polyclonal Antibody, Unconjugated (bs-3818R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
好评度