Anti-Cyclin G2 antibody (bs-3822R)

Anti-Cyclin G2 antibody

DATASHEET

Host:Rabbit

Target Protein:Cyclin G2

IR:Immunogen Range:151-250/344

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:901

Swiss Prot:Q16589

Source:KLH conjugated synthetic peptide derived from human Cyclin G2:151-250/344 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Cyclin G2 may play a role in growth regulation and in negative regulation of cell cycle progesssion. Cyclin G2 expression levels increase through the cell cycle to peak in the mid/late-S phase and decrease during G2/M phase. It may also contribute in maintaining the quiescent state of differentiated cells. Cyclin G2 is similar to cyclin A in the cyclin box, although no kinase activity is detected.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Brain(Rat) lysate at 30ug;
Primary: Anti-Cyclin G2 (bs-3822R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 38kD
Observed band size : 38kD
May be formed of homo-dimer

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin G2) Polyclonal Antibody, Unconjugated (bs-3822R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.