Anti-CYP2R1 antibody (bs-3900R)

Anti-CYP2R1 antibody 

DATASHEET

Host:Rabbit

Target Protein:CYP2R1

IR:Immunogen Range:251-350/501

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:120227

Swiss Prot:Q6VVX0

Source:KLH conjugated synthetic peptide derived from human CYP2R1:251-350/501 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:CYP2R1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are mono-oJNCgenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroJNClase that converts vitamin D into the active ligand for the vitamin D receptor. Defects in CYP2R1 are a cause of 25-hydroJNCvitamin D(3) deficiency, also known as pseudovitamin D(3) deficiency rickets due to 25-hydroJNClase deficiency. First described in patients who had rickets at a young age despite a history of adequate vitamin D intake. The patients sera had low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activity and low levels of 25-hydroJNCvitamin D.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-CYP2R1 (bs-3900R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti- CYP2R1 (bs-3900R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD

Sample:
Liver(Human) Cell Lysate at 40 ug
Primary: Anti-CYP2R1 (bs-3900R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD