Anti-G protein alpha S/NESP55 antibody
| 英文名称 | G protein alpha S/NESP55 |
| 中文名称 | G蛋白αS抗体（鸟嘌呤核苷酸结合蛋白Gα s） |
| 别 名 | Adenylate cyclase stimulating G alpha protein; AHO; Alternative gene product encoded by XL exon; Extra large alphas protein; GNAS; GNAS complex locus; GNAS1; GPSA; Gs alpha subunit; GSA; GSP; Guanine nucleotide binding protein (G protein) alpha stimulating activity polypeptide 1; Guanine nucleotide binding protein alpha stimulating activity polypeptide 1; Guanine nucleotide binding protein G(s) subunit alpha isoforms short; Guanine nucleotide binding protein G(s) subunit alpha isoforms XLas; Guanine nucleotide regulatory protein; MGC33735; NESP; NESP55; Neuroendocrine secretory protein; PHP1A; PHP1B; POH; Protein ALEX; SCG6; Secretogranin VI; XLalphas; XLas; C20orf45; dJ309F20.1.1; dJ806M20.3.3; GNAS1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:G protein alpha S/NESP55
IR:Immunogen Range:901-1037/1037
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2778
Swiss Prot:P63092
Source:KLH conjugated synthetic peptide derived from human G protein alpha S:901-1037/1037
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
Danio rerio.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: tongue(mouse) Lysate at 40 ug
Primary: Anti- G protein alpha S'NESP55 (bs-3939R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111kD
Observed band size: 100 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-G protein alpha S Polyclonal Antibody, Unconjugated(bs-3939R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-G protein alpha S Polyclonal Antibody, Unconjugated(bs-3939R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
好评度