Anti-IGFBP6 antibody (bs-4064R)

Anti-IGFBP6 antibody 

DATASHEET

Host:Rabbit

Target Protein:IGFBP6

IR:Immunogen Range:141-240/240

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3489

Swiss Prot:P24592

Source:KLH conjugated synthetic peptide derived from human IGFBP6:141-240/240 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: muscle (Mouse) Lysate at 40 ug
Primary: Anti- IGFBP6 (bs-4064R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 36 kD

Sample:
Brain (Rat) Lysate at 40 ug
Intestine (Mouse) Lysate at 40 ug
Primary: Anti-IGFBP6 (bs-4064R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IGFBP6 Polyclonal Antibody, Unconjugated(bs-4064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining