Anti-phospho-CD244 (Tyr271) antibody
| 英文名称 | phospho-CD244 (Tyr271) |
| 中文名称 | 磷酸化CD244抗体 |
| 别 名 | CD244(phospho Y271); CD244(phospho-Tyr271); NKR2B4; SLAMF4; natural killer cell receptor 2B4 isoform 2 precursor; 2B4 ; C9.1; CD244 antigen; CD244 molecule natural killer cell receptor 2B4; CD244 natural killer cell receptor 2B4; F730046O15Rik; h2B4; Ly90; NAIL; Natural killer cell activation-inducing ligand; Natural killer cell receptor 2B4; NK cell activation inducing ligand; NK cell activation inducing ligand NAIL; NK cell type I receptor protein 2B4; Nmrk; Non-MHC restricted killing associated; OTTHUMP00000027884; p38; SLAM family, member 4. |
DATASHEET
Host:Rabbit
Target Protein:phospho-CD244 (Tyr271)
IR:Immunogen Range:TI(p-Y)ED
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:51744
Swiss Prot:Q9BZW8
Source:KLH conjugated Synthesised phosphopeptide derived from human CD244 around the phosphorylation site of Tyr271:TI(p-Y)ED
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:CD244 is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. CD244 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness. Although there have been several molecules shown to interact with CD244, the signaling mechanism ofCD244-mediated activation of NK cells is still unknown. It is a high affinity receptor for CD48.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-F(1:100-500)
Flow-Cyt(1ug/Test)
IF(1:100-500)
Cross Reactive Species:Human
Pig
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Hela (Human) Lysate at 40 ug
Primary: Anti- phospho-CD244(Tyr271) (bs-5284R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 65 kD
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-CD244(Tyr271) (bs-5284R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 70 kD
Blank control: U937.
Primary Antibody (green line): Rabbit Anti-CD244 antibody (bs-5284R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
好评度