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Anti-Phospho-CDK1 (Ser39) antibody (bs-5247R)

Anti-Phospho-CDK1 (Ser39) antibody (bs-5247R)

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Anti-Phospho-CDK1 (Ser39) antibody

英文名称Phospho-CDK1 (Ser39)
中文名称磷酸化周期素依赖激酶2抗体
别    名P34CDC2; Cdc 2; cdc2; CDK 1; CDK1; Cell division control protein 2; Cell division control protein 2 homolog; Cell division cycle 2 G1 to S and G2 to M; Cell Divsion Cycle 2 Protein; CDK1_HUMAN.  

DATASHEET

Host:Rabbit

Target Protein:Phospho-CDK1 (Ser39)

IR:Immunogen Range:LE(p-S)EE

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:983

Swiss Prot:P06493

Source:KLH conjugated Synthesised phosphopeptide derived from human cdc2 around the phosphorylation site of Ser39:LE(p-S)EE 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Spleen (Mouse) Lysate at 40 ug
A549(Human) Cell Lysate at 30 ug
Uterus(Mouse) Lysate at 40 ug
Primary: Anti- Phospho-CDK1 (Ser39) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD

Sample:
A431(Human) Cell Lysate at 30 ug
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- Phospho-CDK1 (Ser39) (bs-5247R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD

Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-CDK1 (Ser39) (bs-5247R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD

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