Anti-phospho-HOMER3 (Thr36) antibody
| 英文名称 | phospho-HOMER3 (Thr36) |
| 中文名称 | 磷酸化HOMER3蛋白抗体 |
| 别 名 | HOMER3 (phospho T36); p-HOMER3 (phospho T36); HOMER3 (phospho T36); HOMER3 (phospho Thr36); HOMER 3; Homer 3D protein; Homer homolog 3 (Drosophila); Homer neuronal immediate early gene 3; Homer protein homolog 3; Homer3; VESL3; HOME3_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-HOMER3 (Thr36)
IR:Immunogen Range:AL(p-T)VS
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:9454
Swiss Prot:Q9NSC5
Source:KLH conjugated Synthesised phosphopeptide derived from human HOMER3 around the phosphorylation site of Thr36:AL(p-T)VS
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboJNC-termina coiled-coil domain and two leucine zipper motifs that are involved l in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides. Alternative splicing results in multiple transcript variants.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: A549 Cell (Human) Lysate at 40 ug
Primary: Anti- phospho-HOMER3 (Thr36) (bs-5392R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-HOMER3 (Thr36) (bs-5392R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HOMER3 (Thr36)) Polyclonal Antibody, Unconjugated (bs-5392R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
好评度