Anti-phospho-Tau (Ser199) antibody
| 英文名称 | phospho-Tau (Ser199) |
| 中文名称 | 磷酸化微管相关蛋白抗体 |
| 别 名 | Tau (phospho S199); p-Tau (phospho S199); MAPT(phospho S199); MAPT; Microtuble-associted protein Tau; AI413597; AW045860; DDPAC; Disinhibition dementia parkinsonism amyotrophy complex; FLJ31424; FTDP 17; FTDP17; G Protein beta 1 gamma 2 subunit interacting factor 1; G protein beta1/gamma2 subunit interacting factor 1; MAPTL; MGC134287; MGC138549; MGC156663; Microtubule associated protein tau isoform 4; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament tau; PHF tau; PHF-tau; PPND; pTau; RNPTAU; Tauopathy and respiratory failure, included; TAU_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-Tau (Ser199)
IR:Immunogen Range:YS(P-S)PG
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4137
Swiss Prot:P10636
Source:KLH conjugated Synthesised phosphopeptide derived from human MAPT isoform 2 around the phosphorylation site of Ser199:YS(P-S)PG
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Cerebral cortex (Rat) Lysate at 40 ug
Primary: Anti-phospho-Tau (Ser199) (bs-5417R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52/79 kD
Observed band size: 52/79 kD
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: Cerebellum (Mouse) Lysate at 40 ug
Lane 4: Cerebellum (Rat) Lysate at 40 ug
Primary: Anti-phospho-Tau (Ser199) (bs-5417R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-70 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tau (Ser199)) Polyclonal Antibody, Unconjugated (bs-5417R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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