Anti-Phospho-MAPKAPK2 (Ser272) antibody
| 英文名称 | Phospho-MAPKAPK2 (Ser272) |
| 中文名称 | 磷酸化丝裂原活化蛋白激酶活化的蛋白激酶2抗体 |
| 别 名 | MAPKAP Kinase 2 (phospho S272); p-MAPKAP Kinase 2 (phospho S272);MAPKAPK2(phospho S272);P-MAPKAPK2(Ser272); MAP Kinase Activated Protein Kinase 2; MAPK activated protein kinase 2; MAPKAP kinase 2; MAPKAPK 2; MAPKAPK2; Mitogen Activated Protein Kinase Activated Protein Kinase 2; MAPK2_HUMAN; MK 2; MK2; MAPKAPK-2. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-MAPKAPK2 (Ser272)
IR:Immunogen Range:AI(p-S)PG
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:9261
Swiss Prot:P49137
Source:KLH conjugated Synthesised phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of Ser272:AI(p-S)PG
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:MAP kinase activated protein kinase 2 (MAPKAP Kinase 2), also known as p45 hsp27 kinase, is a 45-54 kDa serine/threonine protein kinase that contains a proline rich sequence and two putative SH3 binding sites. MAPKAP Kinase 2 is activated in response to stress, IL1 and TNF, possibly catalyzed by p38/Hog dependent phosphorylation. One of the major substrates of MAPKAP Kinase 2 is hsp27, which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. MAPKAP2 is implicated in several disorders including ischemic brain injury and heart failure and has been shown to be important in regulating stress resistance and the production of TNF alpha.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-MAPKAPK2(Ser272) (bs-5503R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 47 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-MAPKAPK2(Ser272) Polyclonal Antibody, Unconjugated(bs-5503R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-MAPKAPK2(Ser272) Polyclonal Antibody, Unconjugated(bs-5503R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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