Anti-phospho-NFATc2 (Ser330) antibody
| 英文名称 | phospho-NFATc2 (Ser330) |
| 中文名称 | 磷酸化核因子活化T细胞胞浆蛋白2抗体 |
| 别 名 | NFATC2(phospho Ser330); NFATC2(phospho S330); AI607462; KIAA0611; NF ATp; NF-ATc2; NFAT 1; NFAT pre existing subunit; NFAT transcription complex, preexisting component; NFAT1; NFAT1-D; NFATc2; NFATp; Nuclear factor of activated T cells cytoplasmic 2; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2; Nuclear factor of activated T cells pre-existing component; T cell transcription factor NFAT; NF2IP_HUMAN; Nuclear factor of activated T-cells, cytoplasmic 2; NFAT pre-existing subunit; NF-ATp; T-cell transcription factor NFAT1. |
DATASHEET
Host:Rabbit
Target Protein:phospho-NFATc2 (Ser330)
IR:Immunogen Range:DP(p-S)PV
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4773
Swiss Prot:Q13469
Source:KLH conjugated Synthesised phosphopeptide derived from human NFATc2 around the phosphorylation site of Ser330:DP(p-S)PV
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Spleen (Mouse) Lysate at 40 ug
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- p-NFATc2(Ser330) (bs-5516R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100kD
Observed band size: 110 kD
Sample:
Raji Cell (Human) Lysate at 30 ug
Primary: Anti- p-NFATc2(Ser330) (bs-5516R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100kD
Observed band size: 110 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (phospho-NFATc2(Ser330)) Polyclonal Antibody, Unconjugated (bs-5516R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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