Anti-Collagen IV antibody
| 英文名称 | Collagen IV |
| 中文名称 | IV型胶原蛋白/4型胶原蛋白/胶原蛋白4抗体 |
| 别 名 | Arresten; Canstatin; COL4A1; HANAC; ICH; POREN1; Collagen Alpha 1(IV) Chain; Collagen IV Alpha 1 Polypeptide; Collagen Of Basement Membrane Alpha 1 Chain; Collagen Of Basement Membrane Alpha 2 Chain; Collagen Type IV Alpha 1; DKFZp686I14213; FLJ22259; collagen alpha-1(IV) chain preproprotein; collagen alpha-1(IV) chain preproprotein; Col4a1 protein; collagen of basement membrane, alpha-1 chain; collagen IV, alpha-1 polypeptide; collagen alpha-1(IV) chain; COL4A1 NC1 domain; CO4A1_HUMAN; Collagen Ⅳ; Collagen Type Ⅳ. |
DATASHEET
Host:Rabbit
Target Protein:Collagen IV
IR:Immunogen Range:1571-1669/1669
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1282
Swiss Prot:P02462
Source:KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain:1571-1669/1669
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1:2ug/Test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Collagen IV Polyclonal Antibody, Unconjugated(bs-4595R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Collagen IV Polyclonal Antibody, Unconjugated(bs-4595R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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