Anti-DAL1/EPB41L3 antibody (bs-6003R)

Anti-DAL1/EPB41L3 antibody

DATASHEET

Host:Rabbit

Target Protein:DAL1/EPB41L3

IR:Immunogen Range:131-230/1087

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:23136

Swiss Prot:Q9Y2J2

Source:KLH conjugated synthetic peptide derived from human DAL1/EPB41L3:131-230/1087 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:EPB41L3 is an actin-binding protein originally identified as a molecule whose expression is down-regulated in lung adenocarcinoma. EPB41L3 is a member of the protein 4.1 superfamily, which encompasses structural proteins that play important roles in membrane processes via interactions with actin, spectrin, and the cytoplasmic domains of integral membrane proteins. The gene for EPB41L3 is thought to function as a tumor suppressor and localizes within chromosomal region 18p11.3, which is affected by loss of heterozygosity (LOH) in various adult tumors.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
U-87MG (Human) CellLysate at 30 ug
HepG2 (Human) CellLysate at 30 ug
Primary: Anti-DAL1/EPB41L3 (bs-6003R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 130-140 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAL1(EPB41L3)) Polyclonal Antibody, Unconjugated (bs-6003R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAL1(EPB41L3)) Polyclonal Antibody, Unconjugated (bs-6003R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.