Anti-TdT antibody (bs-2938R)

Anti-TdT antibody

DATASHEET

Host:Rabbit

Target Protein:TdT

IR:Immunogen Range:351-450/509

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1791

Swiss Prot:P04053

Source:KLH conjugated synthetic peptide derived from human TdT/DNTT:351-450/509 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:TdT is a nuclear enzyme involved in the recombination of Immunoglobulin and T cell receptor genes. It is expressed in early lymphoid cells and by tumour cells in certain classes of acute lymphoid leukaemia.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TdT) Polyclonal Antibody, Unconjugated (bs-2938R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-TdT antibody (bs-2938R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.