Anti-Wnt6 antibody (bs-6135R)

Anti-Wnt6 antibody

DATASHEET

Host:Rabbit

Target Protein:Wnt6

IR:Immunogen Range:281-365/365

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7475

Swiss Prot:Q9Y6F9

Source:KLH conjugated synthetic peptide derived from human Wnt6:281-365/365 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Wnt6 is a member of the WNT protein family. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt6 is overexpressed in cervical and breast cell carcinomas and is co-expressed with Wnt10a in colorectal cancer cell lines.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-Wnt6 (bs-6135R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 48 kD

Sample:
MKN45(Human) Cell Lysate at 30 ug
Primary: Anti-Wnt6 (bs-6135R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 42 kD

Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Wnt6 Polyclonal Antibody, Unconjugated(bs-6135R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining