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Anti-PRSS3 antibody (bs-6566R)

Anti-PRSS3 antibody (bs-6566R)

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Anti-PRSS3 antibody 

英文名称PRSS3
中文名称脑胰蛋白酶3抗体
别    名Brain trypsinogen; Mesotrypsin; Mesotrypsinogen; MTG; Pancreatic trypsinogen III; Protease, serine, 3; Protease, serine, 4 (trypsin 4, brain); PRSS3; PRSS4; Serine protease 3; Serine protease 4; T9; TRY3; TRY3_HUMAN; TRY4; Trypsin 3; Trypsin III; Trypsin IV; Trypsin-3; Trypsinogen 4; Trypsinogen 5; Trypsinogen IV.  

DATASHEET

Host:Rabbit

Target Protein:PRSS3

IR:Immunogen Range:101-200/304

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5646

Swiss Prot:P35030

Source:KLH conjugated synthetic peptide derived from human PRSS3:101-200/304 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Function; Digestive protease specialized for the degradation of trypsin inhibitors.
Tissue specificity; Pancreas and brain.
Sequence similarities; Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.
Cellular localization; Secreted.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
A549(Human) Cell Lysate at 40 ug
Primary: Anti-PRSS3 (bs-6566R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRSS3) Polyclonal Antibody, Unconjugated (bs-6566R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRSS3) Polyclonal Antibody, Unconjugated (bs-6566R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Anti-PRSS3 antibody (bs-6566R)

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