Anti-BCAT2 antibody (bs-6589R)

Anti-BCAT2 antibody 

DATASHEET

Host:Rabbit

Target Protein:BCAT2

IR:Immunogen Range:101-200/392

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:587

Swiss Prot:O15382

Source:KLH conjugated synthetic peptide derived from human BCAT2/BCAM:101-200/392 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Class-IV pyridoxal-phosphate-dependent aminotransferase family members ECA39 and BCAT2 are both enzymes that catalyze the first reaction in the catabolism of the essential branched chain amino acids valine, leucine and isoleucine. ECA39, also known as BCAT1 (branched-chain-amino-acid aminotransferase 1, cytosolic) is localized to the cytoplasm where it forms a homodimer. ECA39 is expressed in the brain and kidney during embryogenesis and is overexpressed in c-Myc induced tumors. BCAT2 (branched-chain-amino-acid aminotransferase 2, mitochondrial), also known as placental protein 18 (PP18), is expressed as two isoforms produced by alternative splicing. The first isoform of BCAT2, designated BCAT2A, is expressed in the mitochondrion, while the second isoform, designated BCAT2B, is expressed in the cytoplasm. Ubiquitously expressed, BCAT2 is also thought to act as a transporter of branched chain α-keto acids.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-BCAT2 (bs-6589R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD

Sample: Kidney (Mouse) Tissue Lysate at 30 ug
Primary: Anti-BCAT2(bs-6589R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BCAT2/BCAM Polyclonal Antibody, Unconjugated(bs-6589R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining