Anti-HRH1 antibody (bs-6663R)

Anti-HRH1 antibody 

DATASHEET

Host:Rabbit

Target Protein:HRH1

IR:Immunogen Range:141-240/487

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3269

Swiss Prot:P35367

Source:KLH conjugated synthetic peptide derived from human HRH1:141-240/487 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: U87MG (Human) Cell Lysate at 30 ug
Lane 2: U251 (Human) Cell Lysate at 30 ug
Lane 3: Huvec (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-HRH1 (bs-6663R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Sample:
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti-HRH1 (bs-6663R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD

Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HRH1) Polyclonal Antibody, Unconjugated (bs-6663R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.