Anti-SEMA4A antibody (bs-7519R)

Anti-SEMA4A antibody

DATASHEET

Host:Rabbit

Target Protein:SEMA4A

IR:Immunogen Range:361-460/761

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:64218

Swiss Prot:Q9H3S1

Source:KLH conjugated synthetic peptide derived from human SEMA4A:361-460/761 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:SEMA4A belongs to the semaphorin family of soluble and transmembrane proteins. These proteins play a role in guidance of axonal migration during neuronal development and in immune responses.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-SEMA4A  (bs-7519R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 83 kD

Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-SEMA4A  (bs-7519R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 83 kD

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SEMA4A) Polyclonal Antibody, Unconjugated (bs-7519R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.