Anti-CENPP antibody (bs-7710R)

Anti-CENPP antibody 

DATASHEET

Host:Rabbit

Target Protein:CENPP

IR:Immunogen Range:81-180/288

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:401541

Swiss Prot:Q6IPU0

Source:KLH conjugated synthetic peptide derived from human CENPP:81-180/288 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:CENPP is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:Testis (Mouse)Lysate at 40 ug
Primary: Anti-CENPP(bs-7710R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 33kD
Observed band size: 35kD

Sample:Spleen (Mouse)Lysate at 40 ug
Primary: Anti-CENPP(bs-7710R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 33kD
Observed band size: 36kD

Tissue/cell: human prostate tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CENPP Polyclonal Antibody, Unconjugated(bs-7710R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining