Anti-REC8 antibody (bs-7723R)

Anti-REC8 antibody

DATASHEET

Host:Rabbit

Target Protein:REC8

IR:Immunogen Range:22-120/547

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:9985

Swiss Prot:O95072

Source:KLH conjugated synthetic peptide derived from human REC8:22-120/547 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1ug/Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
Rabbit
Guinea Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: HT29 Cell (Human) Lysate at 40 ug
Primary: Anti-REC8 (bs-7723R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 65 kD

Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-REC8  (bs-7723R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD