Anti-KIFC1 antibody (bs-7805R)

Anti-KIFC1 antibody

DATASHEET

Host:Rabbit

Target Protein:KIFC1

IR:Immunogen Range:401-500/673

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3833

Swiss Prot:Q9BW19

Source:KLH conjugated synthetic peptide derived from human KIFC1:401-500/673 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The kinesins constitute a large family of microtubule-dependent motor proteins, which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell. Individual kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events including endocytosis and transcytosis. KIFC1 is a 673 amino acid protein that belongs to the kinesin-like family of proteins. KIFC1 localizes to the nucleus and contains a C-terminal kinesin-motor domain. Functioning as a minus-end directed microtubule-dependent motor, KIFC1 works together with NuMA and cytoplasmic Dynein to organize microtubule minus ends at spindle poles. HeLa cells deficient in KIFC1 exhibit multipolar mitotic spindles, suggesting that KIFC1 is essential for bipolar spindle formation.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: A549 Cell (Human) Lysate at 40 ug
Primary: Anti-KIFC1 (bs-7805R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 74 kD
Observed band size: 74 kD

Sample: Raji Cell (Human) Lysate at 40 ug
Primary: Anti-KIFC1 (bs-7805R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 74 kD
Observed band size: 74 kD

Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIFC1) Polyclonal Antibody, Unconjugated (bs-7805R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.