Anti-Complement fragment 3c antibody
| 英文名称 | Complement fragment 3c |
| 中文名称 | 补体片段C3c抗体 |
| 别 名 | AHUS5; acylation-stimulating protein cleavage product; ARMD9; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3; CO3_HUMAN; Complement C3; Complement C3c alpha' chain fragment 2; Complement C3c; Complement component 3; Complement component C3; Complement factor 3; CPAMD1. |
DATASHEET
Host:Rabbit
Target Protein:Complement fragment 3c
IR:Immunogen Range:1051-1200/1663
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:718
Swiss Prot:P01024
Source:KLH conjugated synthetic peptide derived from human Complement C3c alpha' chain fragment 1:1051-1200/1663
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Plasma (Rat) Lysate at 40 ug
Primary: Anti-Complement fragment 3c (bs-6416R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 183 kD
Observed band size: 183 kD
Sample:
human serum Lysate at 40 ug
Primary: Anti-Complement fragment 3c (bs-6416R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 183 kD
Observed band size: 183 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Complement fragment 3c) Polyclonal Antibody, Unconjugated (bs-6416R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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