Anti-CCDC30 antibody (bs-8097R)

Anti-CCDC30 antibody

DATASHEET

Host:Rabbit

Target Protein:CCDC30

IR:Immunogen Range:601-700/783

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:728621

Swiss Prot:Q5VVM6

Source:KLH conjugated synthetic peptide derived from human CCDC30:601-700/783 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Expressed in brain, kidney, pancreas, placenta, liver, thymus and prostate.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
HepG2 Cell (Human) Lysate at 40 ug
U251 Cell (Human) Lysate at 40 ug
Primary: Anti-CCDC30 (bs-8097R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kD
Observed band size: 95 kD

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-CCDC30 Polyclonal Antibody, Unconjugated(bs-8097R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC30) Polyclonal Antibody, Unconjugated (bs-8097R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.