Anti-CCDC63 antibody (bs-8137R)

Anti-CCDC63 antibody

DATASHEET

Host:Rabbit

Target Protein:CCDC63

IR:Immunogen Range:251-350/563

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:160762

Swiss Prot:Q8NA47

Source:KLH conjugated synthetic peptide derived from human CCDC63:251-350/563 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:CCDC63 (Coiled-Coil Domain Containing 63) is a Protein Coding gene. An important paralog of this gene is CCDC114.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Siha(Human) Cell Lysate at 40 ug
A549(Human) Cell Lysate at 40 ug
Primary: Anti-CCDC63 (bs-8137R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66/61 kD
Observed band size: 61 kD

Sample:Siha (Human)Cell Lysate at 40 ug
Primary: Anti-CCDC63(bs-8137R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 66kD
Observed band size: 63kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC63) Polyclonal Antibody, Unconjugated (bs-8137R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.