Anti-PDZD8 antibody (bs-9043R)

Anti-PDZD8 antibody

DATASHEET

Host:Rabbit

Target Protein:PDZD8

IR:Immunogen Range:901-1000/1154

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:118987

Swiss Prot:Q8NEN9

Source:KLH conjugated synthetic peptide derived from human PDZD8:901-1000/1154 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:PDZD8 contains a PDZ domain. PDZ domains are protein-interaction domains that are often found in multi-domain scaffolding proteins. The function of this protein remains unknown.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Dog
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Panc-1(Human) Cell Lysate at 30 ug
Primary: Anti-PDZD8 (bs-9043R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 128 kD
Observed band size: 130kD

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-PDZD8 (bs-9043R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 128 kD
Observed band size: 130 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDZD8 Polyclonal Antibody, Unconjugated (bs-9043R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.