Anti-RNF123 antibody (bs-9249R)

Anti-RNF123 antibody

DATASHEET

Host:Rabbit

Target Protein:RNF123

IR:Immunogen Range:1-100/1314

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:63891

Swiss Prot:Q5XPI4

Source:KLH conjugated synthetic peptide derived from human RNF123:1-100/1314 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in protein-protein interactions and protein-DNA interactions. RNF123 (RING finger protein 123), also known as KPC1 (Kip1 (p27) ubiquitination-promoting complex protein 1) or FP1477, contains one RING-type zinc finger domain and one SPRY domain. Localizing to the cytoplasm, RNF123 functions as the catalytic component of the KPC complex that acts as an E3 ubiquitin-protein ligase. Specifically, RNF123 is essential for the ubiquitination and subsequent degradation of p27 during the cell cycle G1 phase. Via its N-terminus, RNF123 is known to interact with GBDR1 (another component of the KPC) and p27 (a cyclin-dependent kinase inhibitor). Due to alternative splicing events, two isoforms exist for RNF123.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-RNF123 (bs-9249R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 149 kD
Observed band size: 149 kD

Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RNF123 Polyclonal Antibody, Unconjugated(bs-9249R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RNF123 Polyclonal Antibody, Unconjugated(bs-9249R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining