Anti-RNF169 antibody (bs-9259R)

Anti-RNF169 antibody

DATASHEET

Host:Rabbit

Target Protein:RNF169

IR:Immunogen Range:401-500/708

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:254225

Swiss Prot:Q8NCN4

Source:KLH conjugated synthetic peptide derived from human RNF169:401-500/708 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:RNF169 contains 1 RING type zinc finger. The exact functions of RNF169 remain unknown.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- RNF169 (bs-9259R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 77 kD
Observed band size: 77 kD

Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- RNF169 (bs-9259R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 77 kD
Observed band size: 77 kD

Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF169) Polyclonal Antibody, Unconjugated (bs-9259R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.