Anti-ERR-alpha antibody
| 英文名称 | ERR-alpha |
| 中文名称 | 雌激素受体相关蛋白α抗体 |
| 别 名 | Estrogen Related Receptor alpha; ERR-α; ERR α; ERRα; ERR alpha; Err 1; ERR a; ERR alpha; ERR-alpha; Err1; ERR1_HUMAN; ERRa; ERRalpha; ESRL 1; ESRL1; ESRR A; Esrra; Estrogen receptor like 1; Estrogen receptor related 1; Estrogen receptor-like 1; Estrogen related receptor alpha; Estrogen-related receptor alpha; Estrra; Nr3b1; Nuclear receptor subfamily 3 group B member 1; Steroid hormone receptor ERR1. |
DATASHEET
Host:Rabbit
Target Protein:ERR-alpha
IR:Immunogen Range:101-200/423
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2101
Swiss Prot:P11474
Source:KLH conjugated synthetic peptide derived from human Estrogen Related Receptor alpha:101-200/423
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lovp(Human) Cell Lysate at 30 ug
Primary: Anti- ERR-alpha (bs-6998R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-ERR-alpha (bs-6998R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERR-alpha) Polyclonal Antibody, Unconjugated (bs-6998R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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