Anti-C3orf17 antibody (bs-9824R)

Anti-C3orf17 antibody 

DATASHEET

Host:Rabbit

Target Protein:C3orf17

IR:Immunogen Range:401-500/567

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:25871

Swiss Prot:Q6NW34

Source:KLH conjugated synthetic peptide derived from human C3orf17:401-500/567 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Chromosome 3 is made up of about 214 million bases encoding over 1,100 genes. Notably, there is a chemokine receptor gene cluster and a variety of human cancer related loci on chromosome 3. Particular regions of the chromosome 3 short arm are deleted in many types of cancer cells. Key tumor suppressing genes on chromosome 3 encode apoptosis mediator RASSF1, cell migration regulator HYAL1 and angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3. The C3orf17 gene product has been provisionally designated C3orf17 pending further characterization.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
IF(1:50-200)
IEM(1:20-200)
IGS(1:20-200)
GICA(1:20-200)

Cross Reactive Species:Human
Mouse
Rat
Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-C3orf17 (bs-9824R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 63 kD

Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti-C3orf17 (bs-9824R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 63 kD

Blank control(blue):RSC96 (fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti- C3orf17 antibody(bs-1638R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.