Anti-phospho-cardiac Troponin I (Thr143) antibody
英文名称 | phospho-cardiac Troponin I (Thr143) |
中文名称 | 磷酸化心肌肌钙蛋白抗体 |
别 名 | cardiac Troponin I (phospho Thr143); p-cardiac Troponin I (Thr143); cardiac Troponin I (phospho T143); p-cardiac Troponin I (T143); Cardiac troponin I; Cardiomyopathy, familial hypertrophic, 7, included; CMD1FF; CMD2A; CMH7; cTnI; Familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; p-Tn1; p-Tni; TNN I3; p-TNNC 1; p-TNNC1; p-TNNI3; p-Troponin I cardiac; Troponin I cardiac muscle; Troponin I cardiac muscle isoform; Troponin I type 3 cardiac; troponin I, cardiac 3; TroponinI; TNNI3_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-cardiac Troponin I (Thr143)
IR:Immunogen Range:RP(p-T)LR
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:7137
Swiss Prot:P19429
Source:KLH conjugated synthesised phosphopeptide derived from human cardiac Troponin I around the phosphorylation site of Thr143:RP(p-T)LR
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq].
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/Test)
IF(1:50-200)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PGGT1B) Polyclonal Antibody, Unconjugated (bs-9860R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-cardiac Troponin I (Thr143)) Polyclonal Antibody, Unconjugated (bs-9860R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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