Anti-EP4 antibody (bs-8538R)

Anti-EP4 antibody

DATASHEET

Host:Rabbit

Target Protein:EP4

IR:Immunogen Range:151-250/488

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5734

Swiss Prot:P35408

Source:KLH conjugated synthetic peptide derived from human EP4R/Prostaglandin E Receptor EP4:151-250/488 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Bee
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-EP4  (bs-8538R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD

Sample:
MCF-7 Cell (Human) Lysate at 30 ug
Primary: Anti-EP4 (bs-8538R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD

Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-EP4 antibody (bs-8538R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.