Anti-TPH2 antibody (bs-8729R)

Anti-TPH2 antibody

DATASHEET

Host:Rabbit

Target Protein:TPH2

IR:Immunogen Range:201-300/490

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:121278

Swiss Prot:Q8IWU9

Source:KLH conjugated synthetic peptide derived from human TPH2:201-300/490 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Phenylalanine hydroJNClase (PAH), tyrosine hydroJNClase (TH), tryptophan hydroJNClase (TPH) and tryptophan hydroJNClase 2 (TPH2) comprise a small family of monooJNCgenases that catalyze the rate-limiting step in the catabolism of aromatic L-amino acids and utilize tetrahydropterine as a cofactor. TPH2 is highly expressed in the central nervous system (CNS), mainly in the brain. TPH2 catalyzes the first step in the biosynthesis of serotonin in the CNS and melatonin in the pineal gland, and may be involved in the pathology of several neuropsychiatric disorders. Glucocorticoid-mediated reduction of TPH2 is associated with the etiology of mood disorders, specifically psychotic major depression, and TPH2 may be related to dysregulation of serotonin neurotransmission in the brain which commonly leads to suicidal behavior.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
U87MG(Human) Cell Lysate at 30 ug
U251(Human) Cell Lysate at 30 ug
Primary: Anti- TPH2 (bs-8729R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 60 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TPH2) Polyclonal Antibody, Unconjugated (bs-8729R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- TPH2 Polyclonal Antibody, Unconjugated(bs-8729R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining