Anti-TREM1 antibody (bs-4886R)

Anti-TREM1 antibody

DATASHEET

Host:Rabbit

Target Protein:TREM1

IR:Immunogen Range:65-150/230

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:58217

Swiss Prot:Q9JKE2

Source:KLH conjugated synthetic peptide derived from mouse TREM1:65-150/230 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1ug/Test)

Cross Reactive Species:Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Liver (Rat) Lysate at 40 ug
Primary: Anti-TREM1 (bs-4886R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 25 kD

Sample:
kidney (Mouse) Lysate at 40 ug
Primary: Anti-TREM1 (bs-4886R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 25 kD

Blank control (black line): Mouse spleen(Black).
Primary Antibody (green line): Rabbit Anti-TREM1 antibody (bs-4886R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% paraformaldehyde for 10 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature The secondary antibody used for 40 min at room temperature. Acquisition of 10,000 events was performed.