Anti-BASP1 antibody (bs-8662R)

Anti-BASP1 antibody

DATASHEET

Host:Rabbit

Target Protein:BASP1

IR:Immunogen Range:3-100/227

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10409

Swiss Prot:P80723

Source:KLH conjugated synthetic peptide derived from human BASP1/Nap22:3-100/227 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Neuronal axonal membrane protein Nap-22, also designated neuronal tissue-enriched acidic protein or brain acid soluble protein (BASP1), is a Ca2+-dependent calmodulin-binding protein that is important for neuronal sprouting and plasticity. Nap-22 is abundant in brain nerve terminals and is also present in significant amounts in kidney, testis and lymphoid tissue. Nap-22 undergoes N-terminal myristoylation for membrane localization. It has been characterized as a major protein of neuronal rafts, which are known to preferentially bind membranes containing cholesterol. Nap-22 is a crucial protein active in neurite outgrowth and synaptic plasticity.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:Hela (Human)Cell Lysate at 40 ug
Primary: Anti-BASP1(bs-8662R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 25kD
Observed band size: 26kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BASP1) Polyclonal Antibody, Unconjugated (bs-8662R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BASP1 Polyclonal Antibody, Unconjugated(bs-8662R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining