Anti-IGF1R antibody (bs-4985R)

Anti-IGF1R antibody 

DATASHEET

Host:Rabbit

Target Protein:IGF1R

IR:Immunogen Range:821-920/1367

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3480

Swiss Prot:P08069

Source:KLH conjugated synthetic peptide derived from human IGF1R/CD221 beta chain:821-920/1367 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:Kidney (Mouse) Lysate at 40 ug
Primary: Anti- IGF1R (bs-4985R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69/150 kD
Observed band size: 69 kD

Blank control(blue): Mouse Spleen(fixed with 2% paraformaldehyde for 10 min at 37℃).
Primary Antibody:Rabbit Anti-IGF1R antibody (bs-4985R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.