Anti-NCR1 antibody (bs-10027R)

Anti-NCR1 antibody

DATASHEET

Host:Rabbit

Target Protein:NCR1

IR:Immunogen Range:198-206/304

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:9437

Swiss Prot:O76036

Source:KLH conjugated synthetic peptide derived from human CD335:198-206/304 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The natural cytotoxicity receptors (NCRs) are a recently characterized family of Ig-like activation receptors that appear to be major triggering receptors in tumor cell recognition. NCR1 is a glycoprotein that has two extracellular Ig-like domains followed by a ~40 amino acid residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. NCR1 has been shown to represent a novel NK cell-specific molecule involved in human NK cell activation. NCR1 has been implicated in NK cell-mediated lysis of several autologous tumor cells and pathogen-infected cell lines.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Kidney (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
A431(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
Primary: Anti- NCR1 (bs-10027R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 46 kD

Sample:
Lane 1: Lymph node (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Lane 3: Lung (Mouse) Lysate at 40 ug
Primary: Anti-NCR1 (bs-10027R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD

Sample:
Lymph node (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti- NCR1 (bs-10027R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 46 kD