Anti-phospho-JMJD2B (Thr305) antibody
| 英文名称 | phospho-JMJD2B (Thr305) |
| 中文名称 | 磷酸化组蛋白去甲基化酶JMJD2B抗体 |
| 别 名 | KDM4B / JMJD2B (phospho Thr305); KDM4B/JMJD2B (phospho T305); KDM4B/JMJD2B (phospho Thr305); p-KDM4B(T305); p-KDM4B(Thr305); JHDM3B; JmjC domain-containing histone demethylation protein 3B; JMJD2B; JmjC domain-containing histone demethylation protein 3A; Jumonji domain containing protein 2B; KIAA0876; Lysine-specific demethylase 4B; KDM4B_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-JMJD2B (Thr305)
IR:Immunogen Range:VA(p-T)QC
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:23030
Swiss Prot:O94953
Source:KLH conjugated synthesised phosphopeptide derived from human JMJD2B around the phosphorylation site of Thr305:VA(p-T)QC
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:JMJD2B is a 1,064 amino acid protein encoded by the human gene JMJD2B. JMJD2B belongs to the JMJD2B histone demethylase family and contains one JmjC domain, one JmjN domain, two PHD-type zinc fingers and two Tudor domains. The two Tudor domains recognize and bind methylated histones and have an interdigitated structure; the unusual fold is required for its ability to bind methylated histone tails. JMJD2B is a histone demethylase that specifically demethylates Lys 9 residues of Histone H3, thereby playing a role in histone code. It does not demethylate Histone H3 Lys 4, H3 Lys 27, H3 Lys 36 or H4 Lys 20, however, and is only able to demethylate trimethylated H3 Lys-9 and has weaker activity than JMJD2A, JMJD2C and JMJD2D. JMJD2B demethyl-ation of Lysine residues will generate formaldehyde and succinate. JMJD2B is a ubiquitously expressed nuclear protein.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-JMJD2B(Thr305) Polyclonal Antibody, Unconjugated(bs-12200R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-JMJD2B(Thr305) Polyclonal Antibody, Unconjugated(bs-12200R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-JMJD2B(Thr305) Polyclonal Antibody, Unconjugated(bs-12200R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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