Anti-14-3-3 Tau antibody
| 英文名称 | 14-3-3 Tau |
| 中文名称 | 14-3-3τ蛋白抗体 |
| 别 名 | 14 3 3 protein T cell; 14 3 3 protein tau; 14 3 3 protein theta; 14 3 3 tau; 14 3 3 theta; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; 1433T_HUMAN; 1C5; HS1; KCIP1; Protein HS1; Protein kinase C inhibitor protein 1; Protein tau; tyr3/trp5 monooJNCgenase activation protein, theta; Tyrosine 3 monooJNCgenase/tryptophan 5 monooJNCgenase activation protein, theta polypeptide; YWHAQ. |
DATASHEET
Host:Rabbit
Target Protein:14-3-3 Tau
IR:Immunogen Range:31-130/245
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:10971
Swiss Prot:P27348
Source:KLH conjugated synthetic peptide derived from human 14-3-3 Tau:31-130/245
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
This antibody is reactive with 14-3-3 Alpha/Beta/Gamma/Delta/Epsilon.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Liver(Rat) Cell Lysate at 40 ug
Primary: Anti-14-3-3 Tau (bs-12419R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (14-3-3 Tau) Polyclonal Antibody, Unconjugated (bs-12419R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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