Anti-phospho-delta 1 Catenin (Thr916) antibody (bs-12991R)

Anti-phospho-delta 1 Catenin (Thr916) antibody 

DATASHEET

Host:Rabbit

Target Protein:phospho-delta 1 Catenin (Thr916)

IR:Immunogen Range:NR(p-T)LD

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1500

Swiss Prot:O60716

Source:KLH conjugated synthesised phosphopeptide derived from human delta 1 Catenin around the phosphorylation site of Thr916:NR(p-T)LD 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene. [provided by RefSeq, Dec 2010]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Large intestine(Mouse) Lysate at 40 ug
Primary: Anti-phospho-delta 1 Catenin (Thr916) (bs-12991R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 108 kD
Observed band size: 108 kD

Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-delta 1 Catenin (Thr916)) Polyclonal Antibody, Unconjugated (bs-12991R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-delta 1 Catenin (Thr916)) Polyclonal Antibody, Unconjugated (bs-12991R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.