Anti-phospho-VAV3 (Tyr141) antibody (bs-10271R)

Anti-phospho-VAV3 (Tyr141) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-VAV3 (Tyr141)

IR:Immunogen Range:DI(p-Y)KG

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10451

Swiss Prot:Q9UKW4

Source:KLH conjugated Synthesised phosphopeptide derived from human VAV3 around the phosphorylation site of Tyr141:DI(p-Y)KG 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The Vav family of Rho guanine nucleotide exchange factors (GEFs) orchestrate signaling events following lymphocyte antigen receptor activation. Vav3, like Vav (also known as Vav1 or p95Vav), undergoes tyrosine phosphorylation downstream of T cell receptor cross-linkage, and subsequently interacts with 2 adaptor molecules, SLP76 and 3BP2. Following these events, however, the paths of Vav and Vav3 diverge; Vav affects IL-2 promotor activity, while Vav3 impacts gene transcription linked to serum response element (SRE). Furthermore, Vav3 expression follows a cell cycle-dependent pattern, with transient upregulation occuring during mitosis. Encforced Vav3 expression leads to the appearance of multinucleate cells, implicating a role for Vav3 in the control of cytokinesis.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum(Mouse) Lysate at 40 ug
Hippocampus(Mouse) Lysate at 40 ug
Primary: Anti-phospho-VAV3 (Tyr141) (bs-10271R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 95 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-VAV3(Tyr141)) Polyclonal Antibody, Unconjugated (bs-10271R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-VAV3 (Tyr141) Polyclonal Antibody, Unconjugated(bs-10271R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining