Anti-Thrombin light chain antibody
| 英文名称 | Thrombin light chain |
| 中文名称 | 凝血酶(凝血因子II)轻链抗体 |
| 别 名 | coagulation factor II; prothrombin; F2; Cf-2; Cf2; FII; F 2; coagulation factor II (thrombin); Coagulation factor II; Coagulation factor II precursor; F2; Factor II; Factor-II; Prothrombin; prothrombin B-chain; PT; serine protease; THRB; THRB_HUMAN; Thrombin; Thrombin light chain. |
DATASHEET
Host:Rabbit
Target Protein:Thrombin light chain
IR:Immunogen Range:328-363/622
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2147
Swiss Prot:P00734
Source:KLH conjugated synthetic peptide derived from human Thrombin light chain:328-363/622
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Thrombin is the final protease in the blood coagulation cascade and serves both pro- and anticoagulant functions through the cleavage of several targets. The ability of thrombin to specifically recognize a wide range of substrates derives from interactions which occur outside of the active site of thrombin. Thrombin possesses two anion binding exosites which mediate many of its interactions with cofactors and substrates, and although many structures of thrombin have been solved, few such interactions have been described in molecular detail. Glycosaminoglycan binding to exosite II of thrombin plays a major role in switching off the procoagulant functions of thrombin by mediating its irreversible inhibition by circulating serpins and by its binding to the endothelial cell surface receptor thrombomodulin.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: placenta (Mouse) Lysate at 40 ug
Primary: Anti-Thrombin light chain(bs-2134R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
Protein: placenta(mouse) lysate at 40ug;
Primary: rabbit Anti-Thrombin light chain (bs-2134R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 64 kD
Observed band size: 64 kD
Protein: liver(mouse) lysate at 40ug;
Primary: rabbit Anti-Thrombin light chain (bs-2134R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 64 kD
Observed band size: 64 kD
好评度