Anti-CRTR1 antibody (bs-12593R)

Anti-CRTR1 antibody

DATASHEET

Host:Rabbit

Target Protein:CRTR1

IR:Immunogen Range:181-280/479

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:29842

Swiss Prot:Q9NZI6

Source:KLH conjugated synthetic peptide derived from human CRTR1:181-280/479 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The long terminal repeat binding protein-1 (LBP-1) mammalian family of transcription factors are proteins that stimulate transcription in HeLa cells from the major late promoter of simian virus 40 in vitro. The two related human LBP-1 genes are TFCP2A, which encodes the alternatively spliced transcripts LBP-1a and LBP-1b, and TFCP2C, which encodes LBP-1c and LBP-1d. LBP-9, also designated transcription factor CP2-like 1 (TFCP2L1), is a protein of 479 amino acids. LBP-9 shows 83% sequence homology with LBP-1b and acts as a suppressor factor inhibiting the stimulation effect of LBP-1b. LBP-9 may regulate the P450scc reporter activity through the -155/-131 element.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: Anti- CRTR1 (bs-12593R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Tissue/cell:human endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CRTR1 Polyclonal Antibody, Unconjugated(bs-12593R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CRTR1 Polyclonal Antibody, Unconjugated(bs-12593R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining