Anti-UBXN2B antibody (bs-13680R)

Anti-UBXN2B antibody

DATASHEET

Host:Rabbit

Target Protein:UBXN2B

IR:Immunogen Range:201-300/331

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:137886

Swiss Prot:Q14CS0

Source:KLH conjugated synthetic peptide derived from human UBXN2B:201-300/331 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The UBX (Ubiquitin regulatory X) domain is an 80 amino acid motif that is usually present on the carboJNC-terminus of certain eukaryotic proteins. UBX domain-containing proteins (UBXD), such as FAF1, p33ING1 and D8S2298E, are typically involved in ubiquitin-related processes. UBXD proteins also constitute the largest family of VCP cofactors and are generally involved in substrate recruitment to VCP, as well as regulation of its activity. UBXD2B (UBX domain-containing protein 2B), also known as NSFL1 cofactor p37 and p97 cofactor p37, is a 331 amino acid protein that contains one UBX domain and one SEP domain. UBXN2B is required for ER and Golgi biogenesis and also plays a role in their maintenance during interphase, as well as their reassembly at the end of mitosis. Through interaction with VCP, UBXN2B forms a complex that has membrane fusion activity.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Adrenal gland (Mouse) Lysate at 40 ug
Primary: Anti-UBXN2B(bs-13680R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBXN2B) Polyclonal Antibody, Unconjugated (bs-13680R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBXN2B) Polyclonal Antibody, Unconjugated (bs-13680R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.